yhdP [2017-8-11 16:0:3]

potential magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation

locus: BSU09550

pI: 4.40

mw: 49.69 kDa

protein length: 444 aa

gene length: 1332 bp

function: export of magnesium

product: potential magnesium efflux pump

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,032,063 → 1,033,397

The protein

Protein family

• UPF0053 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|B2E9D026B668C8530C406353E3400059388566FB|YhdT], [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|YrkA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|YqhB], [protein|A459410312A926365B45CEB4694DE399B38820A2|YugS]

[SW|Domains]

• [SW|DUF21 domain] (5-201)
• 2 [SW|CBS domain]s (220-282, 284-344)

Structure

• [PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|EDFAF3AF6F2E7578733D5BF0295AD614070453F4|yhdQ]-[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]
  description: [Pubmed|22383849]

  regulation

  • threefold induced in the presence of high Mg²⁺ concentrations [Pubmed|31964700]

Biological materials

Mutant

• MGNA-A696 (yhdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/696 NBRP B. subtilis, Japan]

Expression vector

• pGP2931 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 10960106,22383849,25182490,27933050